Transfection:Article Title: Wnt-7a-positive dendritic cytonemes induce synaptogenesis in cortical neurons
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membrane-mCherry (Mattes et al., 2018), pCAG-mGFP membrane-bound GFP (Addgene 14757), pcDNA3.1-Wnt7a-GFP, 7×TRE-SuperTOPFlash-NLS-mCherry , pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene 46295), Lck-mScarlet-I (Addgene 98821), pCS2+-Vhh-CD8-mCherry (cloned from Gal4/LexA-Vhh-CD8-mCherry (Affolter lab) into pCS2+ vector using XhoI/XbaI), pCS2+-GAP43-jGCaMP7s (cloned from pGP-CMV-jGCaMP7s (Addgene 104463) into pCS2+-GAP43-GFP using XbaI/SnaBI), pcDNA3.1-Notum-CD8-mCh (assembled by Gibson cloning from pcDNA3.1-hNotumFL (Gift from J.P Vincent) and pCS2+-Vhh-CD8-mCherry). .. The following primary antibodies were used for immunofluorescence: anti-Wnt7a (abcam; ab100792), anti-LRP6 (BioTechne; FAB1505R), anti-Bassoon (abcam; ab82958), anti-PSD95 (abcam; ab18258), anti-PSD95 (abcam; ab13552), anti-Flotillin-2 (Santa Cruz; sc-28320), anti-Flotillin-2 (abcam; ab113661), anti-Myosin-X (Santa Cruz C-1; sc-166720), anti-Evi (EMD Milipore; YJ5).
Article Title: WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membrane-mCherry , pCAG-mGFP membrane-bound GFP (Addgene # 14757 ), pcDNA3.1-WNT7A-GFP, pcDNA3.2-WNT7A-V5 (Addgene # 43816 ), 7×TRE-SuperTOPFlash-NLS-mCherry , pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene # 46295 ), Lck-mScarlet-I (Addgene # 98821 ), pCS2+-Vhh-CD8-mCherry [cloned from Gal4/LexA-Vhh-CD8-mCherry (M. Affolter, University of Basel, Switzerland) into pCS2+ vector using XhoI/XbaI], pCS2+-GAP43-jGCaMP7 s [cloned from pGP-CMV-jGCaMP7 s (Addgene # 104463 ) into pCS2+-GAP43-GFP using XbaI/SnaBI], pCS2+LRP6-eGFP (gift from G. Davidson, KIT, Karlsruhe, Germany), GFP-Bsn 95-3938 (gift from Eckart Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany), pcDNA3.1-Notum-CD8-mCh [assembled by Gibson cloning with pcDNA3.1-hNotumFL (gift from J. P. Vincent, Francis Crick Institute, London, UK) and pCS2+-Vhh-CD8-mCherry], PSD-95-pTagRFP (Addgene # 52671 ). .. The following primary antibodies were used for immunofluorescence: anti-WNT7A (abcam, ab100792), anti-LRP6 (BioTechne, FAB1505R), anti-bassoon (abcam, ab82958), anti-PSD95 (abcam, ab18258), anti-PSD95 (abcam, ab13552), anti-flotillin 2 (Santa Cruz Biotechnology, sc-28320), anti-flotillin 2 (abcam, ab113661), anti-myosin-X (C-1) (Santa Cruz Biotechnology, sc-166720), anti-Evi (EMD Millipore, YJ5).
Article Title: WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons.
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membranemCherry (Mattes et al., 2018), pCAG-mGFP membrane-bound GFP (Addgene #14757), pcDNA3.1-WNT7A-GFP, pcDNA3.2-WNT7A-V5 (Addgene #43816), 7×TRE-SuperTOPFlash-NLS-mCherry (Moro et al., 2012), pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene #46295), Lck-mScarlet-I (Addgene #98821), pCS2+-Vhh-CD8mCherry [cloned from Gal4/LexA-Vhh-CD8-mCherry (M. Affolter, University of Basel, Switzerland) into pCS2+ vector using XhoI/XbaI], pCS2+-GAP43-jGCaMP7 s [cloned from pGP-CMV-jGCaMP7 s (Addgene #104463) into pCS2+-GAP43-GFP using XbaI/SnaBI], pCS2+LRP6-eGFP (gift from G. Davidson, KIT, Karlsruhe, Germany), GFP-Bsn 95-3938 (gift from Eckart Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany), pcDNA3.1-Notum-CD8-mCh [assembled by Gibson cloning with pcDNA3.1-hNotumFL (gift from J. P. Vincent, Francis Crick Institute, London, UK) and pCS2+-Vhh-CD8-mCherry], PSD-95-pTagRFP (Addgene #52671). .. The following primary antibodies were used for immunofluorescence: anti-WNT7A (abcam, ab100792), anti-LRP6 (BioTechne, FAB1505R), anti-bassoon (abcam, ab82958), anti-PSD95 (abcam, ab18258), antiPSD95 (abcam, ab13552), anti-flotillin 2 (Santa Cruz Biotechnology, sc28320), anti-flotillin 2 (abcam, ab113661), anti-myosin-X (C-1) (Santa Cruz Biotechnology, sc-166720), anti-Evi (EMD Millipore, YJ5).
Membrane:Article Title: Wnt-7a-positive dendritic cytonemes induce synaptogenesis in cortical neurons
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membrane-mCherry (Mattes et al., 2018), pCAG-mGFP membrane-bound GFP (Addgene 14757), pcDNA3.1-Wnt7a-GFP, 7×TRE-SuperTOPFlash-NLS-mCherry , pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene 46295), Lck-mScarlet-I (Addgene 98821), pCS2+-Vhh-CD8-mCherry (cloned from Gal4/LexA-Vhh-CD8-mCherry (Affolter lab) into pCS2+ vector using XhoI/XbaI), pCS2+-GAP43-jGCaMP7s (cloned from pGP-CMV-jGCaMP7s (Addgene 104463) into pCS2+-GAP43-GFP using XbaI/SnaBI), pcDNA3.1-Notum-CD8-mCh (assembled by Gibson cloning from pcDNA3.1-hNotumFL (Gift from J.P Vincent) and pCS2+-Vhh-CD8-mCherry). .. The following primary antibodies were used for immunofluorescence: anti-Wnt7a (abcam; ab100792), anti-LRP6 (BioTechne; FAB1505R), anti-Bassoon (abcam; ab82958), anti-PSD95 (abcam; ab18258), anti-PSD95 (abcam; ab13552), anti-Flotillin-2 (Santa Cruz; sc-28320), anti-Flotillin-2 (abcam; ab113661), anti-Myosin-X (Santa Cruz C-1; sc-166720), anti-Evi (EMD Milipore; YJ5).
Article Title: WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membrane-mCherry , pCAG-mGFP membrane-bound GFP (Addgene # 14757 ), pcDNA3.1-WNT7A-GFP, pcDNA3.2-WNT7A-V5 (Addgene # 43816 ), 7×TRE-SuperTOPFlash-NLS-mCherry , pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene # 46295 ), Lck-mScarlet-I (Addgene # 98821 ), pCS2+-Vhh-CD8-mCherry [cloned from Gal4/LexA-Vhh-CD8-mCherry (M. Affolter, University of Basel, Switzerland) into pCS2+ vector using XhoI/XbaI], pCS2+-GAP43-jGCaMP7 s [cloned from pGP-CMV-jGCaMP7 s (Addgene # 104463 ) into pCS2+-GAP43-GFP using XbaI/SnaBI], pCS2+LRP6-eGFP (gift from G. Davidson, KIT, Karlsruhe, Germany), GFP-Bsn 95-3938 (gift from Eckart Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany), pcDNA3.1-Notum-CD8-mCh [assembled by Gibson cloning with pcDNA3.1-hNotumFL (gift from J. P. Vincent, Francis Crick Institute, London, UK) and pCS2+-Vhh-CD8-mCherry], PSD-95-pTagRFP (Addgene # 52671 ). .. The following primary antibodies were used for immunofluorescence: anti-WNT7A (abcam, ab100792), anti-LRP6 (BioTechne, FAB1505R), anti-bassoon (abcam, ab82958), anti-PSD95 (abcam, ab18258), anti-PSD95 (abcam, ab13552), anti-flotillin 2 (Santa Cruz Biotechnology, sc-28320), anti-flotillin 2 (abcam, ab113661), anti-myosin-X (C-1) (Santa Cruz Biotechnology, sc-166720), anti-Evi (EMD Millipore, YJ5).
Article Title: WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons.
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membranemCherry (Mattes et al., 2018), pCAG-mGFP membrane-bound GFP (Addgene #14757), pcDNA3.1-WNT7A-GFP, pcDNA3.2-WNT7A-V5 (Addgene #43816), 7×TRE-SuperTOPFlash-NLS-mCherry (Moro et al., 2012), pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene #46295), Lck-mScarlet-I (Addgene #98821), pCS2+-Vhh-CD8mCherry [cloned from Gal4/LexA-Vhh-CD8-mCherry (M. Affolter, University of Basel, Switzerland) into pCS2+ vector using XhoI/XbaI], pCS2+-GAP43-jGCaMP7 s [cloned from pGP-CMV-jGCaMP7 s (Addgene #104463) into pCS2+-GAP43-GFP using XbaI/SnaBI], pCS2+LRP6-eGFP (gift from G. Davidson, KIT, Karlsruhe, Germany), GFP-Bsn 95-3938 (gift from Eckart Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany), pcDNA3.1-Notum-CD8-mCh [assembled by Gibson cloning with pcDNA3.1-hNotumFL (gift from J. P. Vincent, Francis Crick Institute, London, UK) and pCS2+-Vhh-CD8-mCherry], PSD-95-pTagRFP (Addgene #52671). .. The following primary antibodies were used for immunofluorescence: anti-WNT7A (abcam, ab100792), anti-LRP6 (BioTechne, FAB1505R), anti-bassoon (abcam, ab82958), anti-PSD95 (abcam, ab18258), antiPSD95 (abcam, ab13552), anti-flotillin 2 (Santa Cruz Biotechnology, sc28320), anti-flotillin 2 (abcam, ab113661), anti-myosin-X (C-1) (Santa Cruz Biotechnology, sc-166720), anti-Evi (EMD Millipore, YJ5).
Cloning:Article Title: Wnt-7a-positive dendritic cytonemes induce synaptogenesis in cortical neurons
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membrane-mCherry (Mattes et al., 2018), pCAG-mGFP membrane-bound GFP (Addgene 14757), pcDNA3.1-Wnt7a-GFP, 7×TRE-SuperTOPFlash-NLS-mCherry , pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene 46295), Lck-mScarlet-I (Addgene 98821), pCS2+-Vhh-CD8-mCherry (cloned from Gal4/LexA-Vhh-CD8-mCherry (Affolter lab) into pCS2+ vector using XhoI/XbaI), pCS2+-GAP43-jGCaMP7s (cloned from pGP-CMV-jGCaMP7s (Addgene 104463) into pCS2+-GAP43-GFP using XbaI/SnaBI), pcDNA3.1-Notum-CD8-mCh (assembled by Gibson cloning from pcDNA3.1-hNotumFL (Gift from J.P Vincent) and pCS2+-Vhh-CD8-mCherry). .. The following primary antibodies were used for immunofluorescence: anti-Wnt7a (abcam; ab100792), anti-LRP6 (BioTechne; FAB1505R), anti-Bassoon (abcam; ab82958), anti-PSD95 (abcam; ab18258), anti-PSD95 (abcam; ab13552), anti-Flotillin-2 (Santa Cruz; sc-28320), anti-Flotillin-2 (abcam; ab113661), anti-Myosin-X (Santa Cruz C-1; sc-166720), anti-Evi (EMD Milipore; YJ5).
Article Title: WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membrane-mCherry , pCAG-mGFP membrane-bound GFP (Addgene # 14757 ), pcDNA3.1-WNT7A-GFP, pcDNA3.2-WNT7A-V5 (Addgene # 43816 ), 7×TRE-SuperTOPFlash-NLS-mCherry , pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene # 46295 ), Lck-mScarlet-I (Addgene # 98821 ), pCS2+-Vhh-CD8-mCherry [cloned from Gal4/LexA-Vhh-CD8-mCherry (M. Affolter, University of Basel, Switzerland) into pCS2+ vector using XhoI/XbaI], pCS2+-GAP43-jGCaMP7 s [cloned from pGP-CMV-jGCaMP7 s (Addgene # 104463 ) into pCS2+-GAP43-GFP using XbaI/SnaBI], pCS2+LRP6-eGFP (gift from G. Davidson, KIT, Karlsruhe, Germany), GFP-Bsn 95-3938 (gift from Eckart Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany), pcDNA3.1-Notum-CD8-mCh [assembled by Gibson cloning with pcDNA3.1-hNotumFL (gift from J. P. Vincent, Francis Crick Institute, London, UK) and pCS2+-Vhh-CD8-mCherry], PSD-95-pTagRFP (Addgene # 52671 ). .. The following primary antibodies were used for immunofluorescence: anti-WNT7A (abcam, ab100792), anti-LRP6 (BioTechne, FAB1505R), anti-bassoon (abcam, ab82958), anti-PSD95 (abcam, ab18258), anti-PSD95 (abcam, ab13552), anti-flotillin 2 (Santa Cruz Biotechnology, sc-28320), anti-flotillin 2 (abcam, ab113661), anti-myosin-X (C-1) (Santa Cruz Biotechnology, sc-166720), anti-Evi (EMD Millipore, YJ5).
Article Title: WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons.
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membranemCherry (Mattes et al., 2018), pCAG-mGFP membrane-bound GFP (Addgene #14757), pcDNA3.1-WNT7A-GFP, pcDNA3.2-WNT7A-V5 (Addgene #43816), 7×TRE-SuperTOPFlash-NLS-mCherry (Moro et al., 2012), pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene #46295), Lck-mScarlet-I (Addgene #98821), pCS2+-Vhh-CD8mCherry [cloned from Gal4/LexA-Vhh-CD8-mCherry (M. Affolter, University of Basel, Switzerland) into pCS2+ vector using XhoI/XbaI], pCS2+-GAP43-jGCaMP7 s [cloned from pGP-CMV-jGCaMP7 s (Addgene #104463) into pCS2+-GAP43-GFP using XbaI/SnaBI], pCS2+LRP6-eGFP (gift from G. Davidson, KIT, Karlsruhe, Germany), GFP-Bsn 95-3938 (gift from Eckart Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany), pcDNA3.1-Notum-CD8-mCh [assembled by Gibson cloning with pcDNA3.1-hNotumFL (gift from J. P. Vincent, Francis Crick Institute, London, UK) and pCS2+-Vhh-CD8-mCherry], PSD-95-pTagRFP (Addgene #52671). .. The following primary antibodies were used for immunofluorescence: anti-WNT7A (abcam, ab100792), anti-LRP6 (BioTechne, FAB1505R), anti-bassoon (abcam, ab82958), anti-PSD95 (abcam, ab18258), antiPSD95 (abcam, ab13552), anti-flotillin 2 (Santa Cruz Biotechnology, sc28320), anti-flotillin 2 (abcam, ab113661), anti-myosin-X (C-1) (Santa Cruz Biotechnology, sc-166720), anti-Evi (EMD Millipore, YJ5).
Clone Assay:Article Title: WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membrane-mCherry , pCAG-mGFP membrane-bound GFP (Addgene # 14757 ), pcDNA3.1-WNT7A-GFP, pcDNA3.2-WNT7A-V5 (Addgene # 43816 ), 7×TRE-SuperTOPFlash-NLS-mCherry , pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene # 46295 ), Lck-mScarlet-I (Addgene # 98821 ), pCS2+-Vhh-CD8-mCherry [cloned from Gal4/LexA-Vhh-CD8-mCherry (M. Affolter, University of Basel, Switzerland) into pCS2+ vector using XhoI/XbaI], pCS2+-GAP43-jGCaMP7 s [cloned from pGP-CMV-jGCaMP7 s (Addgene # 104463 ) into pCS2+-GAP43-GFP using XbaI/SnaBI], pCS2+LRP6-eGFP (gift from G. Davidson, KIT, Karlsruhe, Germany), GFP-Bsn 95-3938 (gift from Eckart Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany), pcDNA3.1-Notum-CD8-mCh [assembled by Gibson cloning with pcDNA3.1-hNotumFL (gift from J. P. Vincent, Francis Crick Institute, London, UK) and pCS2+-Vhh-CD8-mCherry], PSD-95-pTagRFP (Addgene # 52671 ). .. The following primary antibodies were used for immunofluorescence: anti-WNT7A (abcam, ab100792), anti-LRP6 (BioTechne, FAB1505R), anti-bassoon (abcam, ab82958), anti-PSD95 (abcam, ab18258), anti-PSD95 (abcam, ab13552), anti-flotillin 2 (Santa Cruz Biotechnology, sc-28320), anti-flotillin 2 (abcam, ab113661), anti-myosin-X (C-1) (Santa Cruz Biotechnology, sc-166720), anti-Evi (EMD Millipore, YJ5).
Article Title: WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons.
Article Snippet: .. The following plasmids were used in transfections: pCS2+-membranemCherry (Mattes et al., 2018), pCAG-mGFP membrane-bound GFP (Addgene #14757), pcDNA3.1-WNT7A-GFP, pcDNA3.2-WNT7A-V5 (Addgene #43816), 7×TRE-SuperTOPFlash-NLS-mCherry (Moro et al., 2012), pCS2+-LifeAct-GFP, pCAG-PSD95.FingR-eGFP-CCR5TC (Addgene #46295), Lck-mScarlet-I (Addgene #98821), pCS2+-Vhh-CD8mCherry [cloned from Gal4/LexA-Vhh-CD8-mCherry (M. Affolter, University of Basel, Switzerland) into pCS2+ vector using XhoI/XbaI], pCS2+-GAP43-jGCaMP7 s [cloned from pGP-CMV-jGCaMP7 s (Addgene #104463) into pCS2+-GAP43-GFP using XbaI/SnaBI], pCS2+LRP6-eGFP (gift from G. Davidson, KIT, Karlsruhe, Germany), GFP-Bsn 95-3938 (gift from Eckart Gundelfinger, Leibniz Institute for Neurobiology, Magdeburg, Germany), pcDNA3.1-Notum-CD8-mCh [assembled by Gibson cloning with pcDNA3.1-hNotumFL (gift from J. P. Vincent, Francis Crick Institute, London, UK) and pCS2+-Vhh-CD8-mCherry], PSD-95-pTagRFP (Addgene #52671). .. The following primary antibodies were used for immunofluorescence: anti-WNT7A (abcam, ab100792), anti-LRP6 (BioTechne, FAB1505R), anti-bassoon (abcam, ab82958), anti-PSD95 (abcam, ab18258), antiPSD95 (abcam, ab13552), anti-flotillin 2 (Santa Cruz Biotechnology, sc28320), anti-flotillin 2 (abcam, ab113661), anti-myosin-X (C-1) (Santa Cruz Biotechnology, sc-166720), anti-Evi (EMD Millipore, YJ5).
|
Addgene inc is a verified supplier 